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Hprttm73(Ple142-lacZ)Ems
Targeted Allele Detail
Nomenclature
Symbol: Hprttm73(Ple142-lacZ)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 73, Elizabeth M Simpson
MGI ID: MGI:4835011
Synonyms: Hprttm73(mEMS4751)Ems, NR2E1-lacZ, Ple142
Gene: Hprt  Location: ChrX:52988137-53021659 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Lines:  mEMS4748, mEMS4749, mEMS4750, mEMS4751
Germline Transmission:  Earliest citation of germline transmission: J:145689
Parent Cell Line:  mEMS1202 (ES Cell)
Strain of Origin:  (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion     Vector Type: Not Applicable
 
Mutation detailsThe Ple142-lacZ transgene (bEMS86) was designed with the Ple142 MaxiPromoter (NR2E1-X; derived from the BAC RP11-144P8 that contains the whole (NR2E1) gene plus ~42 kb upstream and ~52 kb downstream, and also contains a portion of the SNX3 gene in the downstream sequence from the nuclear receptor subfamily 2, group E, member 1 (NR2E1) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. (J:144244, J:164356)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1272 strains or lines available
Notes
Germ line transmission of mutant cell line mEMS4751 has been established.
References
Original:  J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/06/2021
MGI 6.16
The Jackson Laboratory