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Hprttm43(Ple140-lacZ)Ems
Targeted Allele Detail
Nomenclature
Symbol: Hprttm43(Ple140-lacZ)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 43, Elizabeth M Simpson
MGI ID: MGI:4834856
Synonyms: Hprttm43(mEMS4485)Ems, NR21E1-F-lacZ, Ple140
Gene: Hprt  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Lines:  mEMS4485, mEMS4486
Germline Transmission:  Earliest citation of germline transmission: J:145689
Parent Cell Line:  mEMS1202 (ES Cell)
Strain of Origin:  (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion     Vector Type: Not Applicable
 
Mutation detailsThe Ple140-lacZ transgene (pEMS1612) was designed with the 4379 bp Ple140 minipromoter (NR2E1-F; derived from a portion of the promoter of the NR2E1 gene and five upstream putative regulatory elements from the Orphan nuclear receptor NR2E1 (NR2E1) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. (J:144244, J:164356)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1274 strains or lines available
Notes
Germ line transmission of mutant cell line mEMS4485 has been established.
References
Original:  J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/16/2021
MGI 6.17
The Jackson Laboratory