About   Help   FAQ
Hprttm42(Ple131-lacZ)Ems
Targeted Allele Detail
Nomenclature
Symbol: Hprttm42(Ple131-lacZ)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 42, Elizabeth M Simpson
MGI ID: MGI:4834828
Synonyms: Hprttm42(mEMS4655)Ems, MKI67-D-lacZ, Ple131
Gene: Hprt  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Lines:  mEMS4655, mEMS4657, mEMS4658, mEMS4659
Germline Transmission:  Earliest citation of germline transmission: J:145689
Parent Cell Line:  mEMS1202 (ES Cell)
Strain of Origin:  (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion     Vector Type: Not Applicable
 
Mutation detailsThe Ple131-lacZ transgene (pESM1602) was designed with the 3088 bp Ple131 minipromoter (MKI67-D; derived from two upstream and one downstream putative regulatory subregions along with a section of the promoter from the human antigen identified by monoclonal antibody Ki-67 (MKI67) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. (J:144244, J:164356)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1274 strains or lines available
Notes
Germ line transmission of mutant cell line mEMS4655 has been established.
References
Original:  J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
09/14/2021
MGI 6.17
The Jackson Laboratory