About   Help   FAQ
Hprttm37(lacZ)Ems
Targeted Allele Detail
Nomenclature
Symbol: Hprttm37(lacZ)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 37, Elizabeth M Simpson
MGI ID: MGI:4834777
Synonyms: Hprttm37(mEMS3689)Ems, Ptomoterless-lacZ-mEMA3689
Gene: Hprt  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Lines:  mEMS3684, mEMS3689, mEMS3768, mEMS3770
Germline Transmission:  Earliest citation of germline transmission: J:145689
Parent Cell Line:  mEMS1202 (ES Cell)
Strain of Origin:  (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion     Vector Type: Not Applicable
 
Mutation detailsThe "Promoterless-lacZ" transgene (pEMS1313) was designed with no Pleiades minipromoter upstream of a frt-flanked _-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. Without a promoter directing expression of beta-galactosidase (lacZ), no lacZ expression pattern is seen or anticipated. (J:144244, J:164356)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1274 strains or lines available
Notes
Germ line transmission of mutant cell line mEMS3689 has been established.
References
Original:  J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
11/16/2021
MGI 6.17
The Jackson Laboratory