Gt(ROSA)26Sortm32.1(CAG-COP4*H134R/EYFP)Hze
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm32.1(CAG-COP4*H134R/EYFP)Hze |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 32.1, Hongkui Zeng |
MGI ID: |
MGI:4834652 |
Synonyms: |
Ai32DeltaNeo, Ai32D (Rosa-CAG-LSL-ChR2(H134R)-EYFP-WPRE), Rosa26-LSL-ChR2-YFP |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm32.1(CAG-COP4*H134R/EYFP)Hze page
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Allele Type: |
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Targeted (Inserted expressed sequence, Reporter) |
Mutation: |
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Insertion
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Mutation details: The Rosa-CAG-LSL-ChR2(H134R)-EYFP-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), a ChR2(H134R)-EYFP fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. To create the ChR2(H134R)-EYFP fusion gene, a cDNA sequence encoding the first 315 amino acids of channelrhodopsin-2 (derived from the green alga Chlamydomonas reinhardtii) was modified with a gain-of-function H134R substitution (CAC to CGC) designed to cause larger stationary photocurrents. This ChR2(H134R) sequence was fused in-frame to the amino terminus of an enhanced yellow fluorescent protein sequence, resulting in the final ChR2(H134R)-EYFP fusion protein sequence. This entire construct was inserted between exons 1 and 2 of the locus. PhiC31 mediated recombination removed the PGK-FRT-Neo-polyA cassette.
(J:155793)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gt(ROSA)26Sor Mutation: |
942 strains or lines available
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Original: |
J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40 |
All: |
12 reference(s) |
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