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Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 27.1, Hongkui Zeng
MGI ID: MGI:4834650
Synonyms: Ai27, Ai27DeltaNeo, Ai27D (Rosa-CAG-LSL-hChR2(H134R)-tdTomato-WPRE), ChR2(H134R)-tdTomato, Rosa26-lsl-ChR2-tdTomato
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:182204
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
 
Mutation detailsThe Rosa-CAG-LSL-hChR2(H134R)-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), a mammalianized ChR2(H134R)-tdTomato fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. To create the mammalianized ChR2(H134R)-tdTomato fusion gene, a cDNA sequence encoding the first 315 amino acids of channelrhodopsin-2 (derived from the green alga Chlamydomonas reinhardtii) was modified to harbor condons optimized for mammalian expression and a gain-of-function H134R substitution (CAC to CGC) designed to cause larger stationary photocurrents. This mhChR2 sequence (also called hChR2(H134R) or hChR2-H134R) was fused in-frame to the amino terminus of a tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), resulting in the final hChR2(H134R)-tdTomato fusion protein sequence. The entire Rosa-CAG-LSL-hChR2(H134R)-tdTomato-WPRE targeting vector was inserted between exons 1 and 2 of the locus. PhiC31 mediated recombination removed the PGK-FRT-Neo-polyA cassette. (J:182204)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  538 strains or lines available
References
Original:  J:182204 Madisen L, et al., A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nat Neurosci. 2012 Mar 25;15(5):793-802
All:  46 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/15/2020
MGI 6.15
The Jackson Laboratory