Tg(Thy1-cre/ERT2,-EYFP)HGfng
Transgene Detail

Summary

• Symbol: Tg(Thy1-cre/ERT2,-EYFP)HGfng
• Name: transgene insertion H, Guoping Feng
• MGI ID: MGI:4830789
• Synonyms: SLICK-H
• Transgene: Tg(Thy1-cre/ERT2,-EYFP)HGfng Location: unknown
• Alliance: Tg(Thy1-cre/ERT2,-EYFP)HGfng page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Thy1-cre/ERT2,-EYFP)HGfng expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Thy1 (MGI:98747)

• Mutation details: The transgene was designed with a creERT2 fusion gene, two copies of the mouse Thy1 promoter, and an enhanced yellow fluorescent protein (EYFP) cDNA sequence. The creERT2 fusion gene (cre/ERT2) is cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand-binding domain. Each Thy1 promoter region is a modified regulatory region of the murine thy1.2 gene (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns). The two copies are placed "back to back" to function bidirectionally; one copy drives expression of CreERT2, the other copy drives expression of EYFP. Founder line H (SLICK-H) was subsequently established and backcrossed to C57BL/6J for 4 generations. (J:134019)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Thy1 (mouse) Summary of all recombinase alleles driven by Thy1. Alliance mouse Thy1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:122791 Deisseroth K, et al., Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci. 2006 Oct 11;26(41):10380-6
• All: 43 reference(s)