Cdh1^tm1.1Mpst
Targeted Allele Detail

Summary

• Symbol: Cdh1^tm1.1Mpst
• Name: cadherin 1; targeted mutation 1.1, Marc P Stemmler
• MGI ID: MGI:4821988
• Synonyms: Cdh1^Cdh1-HA, Cdh1^HA
• Gene: Cdh1 Location: Chr8:107329983-107396878 bp, + strand Genetic Position: Chr8, 53.18 cM, cytoband D
• Alliance: Cdh1^tm1.1Mpst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163273
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: A C terminally HA tagged mouse Cdh1 cDNA and an inverted floxed TK-neo cassette were inserted in the start codon via homologous recombination. The neo cassette was then removed by cre mediated recombination. RNA and protein levels are reduced in homozygous ES cells relative to wild-type cells. Immunoprecipitation assays confirmed the ability of the HA tagged protein to interact with normal binding partners. Expression analysis found loss of protein expression restricted to the labyrinth layer of the placenta. (J:163273)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdh1 Mutation: 171 strains or lines available

References

• Original: J:163273 Stemmler MP, et al., A Cdh1HA knock-in allele rescues the Cdh1-/- phenotype but shows essential Cdh1 function during placentation. Dev Dyn. 2010 Sep;239(9):2330-44
• All: 1 reference(s)