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Cdh1tm1.1Mpst
Targeted Allele Detail
Nomenclature
Symbol: Cdh1tm1.1Mpst
Name: cadherin 1; targeted mutation 1.1, Marc P Stemmler
MGI ID: MGI:4821988
Synonyms: Cdh1Cdh1-HA, Cdh1HA
Gene: Cdh1  Location: Chr8:107329983-107396878 bp, + strand  Genetic Position: Chr8, 53.18 cM, cytoband D
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:163273
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Hypomorph, Reporter)
Mutation:    Insertion
 
Mutation detailsA C terminally HA tagged mouse Cdh1 cDNA and an inverted floxed TK-neo cassette were inserted in the start codon via homologous recombination. The neo cassette was then removed by cre mediated recombination. RNA and protein levels are reduced in homozygous ES cells relative to wild-type cells. Immunoprecipitation assays confirmed the ability of the HA tagged protein to interact with normal binding partners. Expression analysis found loss of protein expression restricted to the labyrinth layer of the placenta. (J:163273)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cdh1 Mutation:  166 strains or lines available
References
Original:  J:163273 Stemmler MP, et al., A Cdh1HA knock-in allele rescues the Cdh1-/- phenotype but shows essential Cdh1 function during placentation. Dev Dyn. 2010 Sep;239(9):2330-44
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/10/2022
MGI 6.19
The Jackson Laboratory