Prkaca^tm4.1Gsm
Targeted Allele Detail

Summary

• Symbol: Prkaca^tm4.1Gsm
• Name: protein kinase, cAMP dependent, catalytic, alpha; targeted mutation 4.1, G Stanley McKnight
• MGI ID: MGI:4821830
• Synonyms: CalphaM120A
• Gene: Prkaca Location: Chr8:84699622-84723072 bp, + strand Genetic Position: Chr8, 40.22 cM, cytoband C3
• Alliance: Prkaca^tm4.1Gsm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163375
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A floxed neomycin selection cassette, a cDNA containing exons 5-10, and a third loxP site were all inserted in intron 4. The endogenous exon 5 was replaced with one encoding an M120A mutation. The floxed neo and exons 5-10 minigene were excised by cre resulting in expression of the mutated exon 5. (J:163375)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkaca Mutation: 22 strains or lines available

References

• Original: J:163375 Morgan DJ, et al., Tissue-specific PKA inhibition using a chemical genetic approach and its application to studies on sperm capacitation. Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20740-5
• All: 1 reference(s)