Tg(CRYD/Gli-luc)#Ech
Transgene Detail

Summary

• Symbol: Tg(CRYD/Gli-luc)#Ech
• Name: transgene insertion, Eric C Holland
• MGI ID: MGI:4820828
• Transgene: Tg(CRYD/Gli-luc)#Ech Location: unknown
• Alliance: Tg(CRYD/Gli-luc)#Ech page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: Mice bearing this transgene express luciferase from a promoter that is activated by glioma-associated oncogene proteins (GLI), primary targets of sonic hedgehog signaling. The transgene is derived from the pGL3b/8GliBS-luc plasmid, which contains eight copies of the human GLI-binding DNA consensus sequence (5'-GAACACCCA-3') upstream of the chicken lens delta-crystallin (ASL/CRYD) basal promoter driving expression of the firefly luciferase gene. The transgene serves as a reporter of GLI activity based on bioluminescence (BLI) following local or systemic luciferin injection. Nine founders with germline transmission were found; granule cell preparations from only one line displayed tight regulation of expression. Transgenic line numbers are not provided. (J:133329)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:133329 Becher OJ, et al., Gli activity correlates with tumor grade in platelet-derived growth factor-induced gliomas. Cancer Res. 2008 Apr 1;68(7):2241-9
• All: 4 reference(s)