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Lta4htm1a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Lta4htm1a(KOMP)Wtsi
Name: leukotriene A4 hydrolase; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4820073
Gene: Lta4h  Location: Chr10:93453411-93484875 bp, + strand  Genetic Position: Chr10, 48.42 cM, cytoband C3
Mutation
origin
Mutant Cell Lines:  EPD0612_5_A01, EPD0612_5_A02, EPD0612_5_A03, EPD0612_5_B01, EPD0612_5_B04, EPD0612_5_C01, EPD0612_5_C02, EPD0612_5_C04, EPD0612_5_D01, EPD0612_5_D02, EPD0612_5_E01, EPD0612_5_E02, EPD0612_5_E03, EPD0612_5_E04, EPD0612_5_F01, EPD0612_5_F02, EPD0612_5_F03, EPD0612_5_F04, EPD0612_5_G03, EPD0612_5_G04, EPD0612_5_H04
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 93470069 of Chromosome 10 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 93471516. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 1 line available
Carrying any Lta4h Mutation:  18 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/19/2021
MGI 6.16
The Jackson Laboratory