Pcbp2^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: Pcbp2^tm1(KOMP)Mbp
• Name: poly(rC) binding protein 2; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4820015
• Gene: Pcbp2 Location: Chr15:102378974-102408496 bp, + strand Genetic Position: Chr15, 57.61 cM, cytoband F1
• Alliance: Pcbp2^tm1(KOMP)Mbp page
• IMPC: Pcbp2 gene page

Mutation origin

• Mutant Cell Lines: DEPD00527_4_A05, DEPD00527_4_B08, DEPD00527_4_C05, DEPD00527_4_C06, DEPD00527_4_C07, DEPD00527_4_C08, DEPD00527_4_D06, DEPD00527_4_D07, DEPD00527_4_D08, DEPD00527_4_E06, DEPD00527_4_E07, DEPD00527_4_E08, DEPD00527_4_F07, DEPD00527_4_G05, DEPD00527_4_G06, DEPD00527_4_G07, DEPD00527_4_H05, DEPD00527_4_H07
• Germline Transmission: Earliest citation of germline transmission: J:233719
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 1905 starting at position 102381333 and ending at position 102383238 of Chromosome 15 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064, J:233719)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Pcbp2 Mutation: 60 strains or lines available

Notes

Germline transmission was made in mutant cell line DEPD00527_4_A05 (J:233719).

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 2 reference(s)