A730085E03Rik^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: A730085E03Rik^tm1(KOMP)Mbp
• Name: RIKEN cDNA A730085E03 gene; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4820003
• Gene: A730085E03Rik Location: Chr18:69328861-69332203 bp, + strand Genetic Position: Chr18, 43.02 cM
• IMPC: A730085E03Rik gene page

Mutation origin

• Mutant Cell Lines: DEPD00528_4_A06, DEPD00528_4_A07, DEPD00528_4_A08, DEPD00528_4_B06, DEPD00528_4_C06, DEPD00528_4_C07, DEPD00528_4_C08, DEPD00528_4_G06, DEPD00528_4_H06
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 709 starting at position 69331503 and ending at position 69332212 of Chromosome 18 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any A730085E03Rik Mutation: 1 strain or line available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 1 reference(s)