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Tg(Cspg4-cre/Esr1*)BAkik
Transgene Detail
Nomenclature
Symbol: Tg(Cspg4-cre/Esr1*)BAkik
Name: transgene insertion B, Akiko Nishiyama
MGI ID: MGI:4819178
Synonyms: NG2-CreER, NG2-CreER, NG2creERB, NG2creERTM, NG2creERTM
Transgene: Tg(Cspg4-cre/Esr1*)BAkik  Location: Chr14:106654779-106655407 bp  Genetic Position: Chr14, Syntenic
Transgene
origin
Strain of Origin:  C57BL/6 or (C57BL/6 x SJL)F1
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intergenic deletion
 
Mutation detailsThe 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), was modified by inserting a CreERTM fusion gene (cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) followed by a rabbit beta-globin polyA signal, into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the CreERTM sequence. The ~219 kb BAC sequence was linearized and microinjected into the pronucleus of fertilized oocytes from C57BL/6 or (C57BL/6 x SJL)F1 mice. Mice derived from line B (NG2creERB) had no background cre activity in the absence of tamoxifen, with cre activity specifically in NG2-expressing cells after tamoxifen injection. Transgene insertion occurred on Chr 14, causing a 628 bp deletion. (J:162529, J:167902, J:253473)
Recombinase
activity
Activity:
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Driver: Cspg4     Summary of all recombinase alleles driven by Cspg4. Comparative matrix view of recombinase activities
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:167902 Zhu X, et al., Age-dependent fate and lineage restriction of single NG2 cells. Development. 2011 Feb;138(4):745-53
All:  62 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
12/10/2019
MGI 6.14
The Jackson Laboratory