Bmp4^tm5.1Bhr
Targeted Allele Detail

Summary

• Symbol: Bmp4^tm5.1Bhr
• Name: bone morphogenetic protein 4; targeted mutation 5.1, Richard R Behringer
• MGI ID: MGI:4460795
• Synonyms: Bmp4^CFP
• Gene: Bmp4 Location: Chr14:46620982-46628126 bp, - strand Genetic Position: Chr14, 23.95 cM
• Alliance: Bmp4^tm5.1Bhr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159629
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A floxed Pgk-neo-bpA cassette was inserted into the Eco RI site into the preserved third intron in reverse orientation. An improved YFP, Citrine, coding sequence was inserted into the fourth exon as an in-frame fusion after the furin cleavage site that generates the mature peptide. The floxed Neo cassette was removed through crossing with Sox2-Cre transgenic animals. Although no endogenous sequence was deleted, this allele was determined to be functionally null allele. (J:159629)

Expression

In Mice Carrying this Mutation:

27 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bmp4 Mutation: 21 strains or lines available

References

• Original: J:159629 Jang CW, et al., Bmp4-directed nuclear cyan fluorescent protein provides a tool for live imaging and reveals cellular resolution of Bmp4 expression patterns during embryogenesis. Int J Dev Biol. 2010;54(5):931-8
• All: 3 reference(s)