Dact1^tm1.3Bnrc
Targeted Allele Detail

Summary

• Symbol: Dact1^tm1.3Bnrc
• Name: dishevelled-binding antagonist of beta-catenin 1; targeted mutation 1.3, Benjamin N R Cheyette
• MGI ID: MGI:4460656
• Gene: Dact1 Location: Chr12:71356658-71366881 bp, + strand Genetic Position: Chr12, 29.39 cM
• Alliance: Dact1^tm1.3Bnrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152701
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An frt flanked neomycin resistance (neo) cassette was inserted upstream of exon 2 and exon 2 was floxed via homologous recombination. Cre-mediated recombination then removed exon 2 and Flp recombinase was used to delete the neomycin selection cassette. (J:152701)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dact1 Mutation: 18 strains or lines available

Notes

Dr. Cheyette indicates that they did look at the phenotype of Dact1^tm1.3Bnrc and as far as they could tell it was the same as for Dact1^tm1.1Bnrc

References

• Original: J:152701 Suriben R, et al., Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak. Nat Genet. 2009 Sep;41(9):977-85
• All: 2 reference(s)