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Ap5z1tm1(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Ap5z1tm1(KOMP)Wtsi
Name: adaptor-related protein complex 5, zeta 1 subunit; targeted mutation 1, Wellcome Trust Sanger Institute
MGI ID: MGI:4460281
Synonyms: AP5 zeta KO
Gene: Ap5z1  Location: Chr5:142449699-142464465 bp, + strand  Genetic Position: Chr5, 81.53 cM
Mutation
origin
Mutant Cell Lines:  EPD0587_5_A05, EPD0587_5_A06, EPD0587_5_A08, EPD0587_5_B06, EPD0587_5_B07, EPD0587_5_B08, EPD0587_5_C08, EPD0587_5_D08, EPD0587_5_E06, EPD0587_5_E07, EPD0587_5_E08, EPD0587_5_F06, EPD0587_5_G06, EPD0587_5_H05, EPD0587_5_H06, EPD0587_5_H07
Germline Transmission:  Earliest citation of germline transmission: J:283611
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe insertion of the L1L2_Bact_P cassette created a deletion of size 3956 starting at position 142451972 and ending at position 142455928 of Chromosome 5 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Ap5z1 Mutation:  32 strains or lines available
Notes
Cell line EPD0587_5_A05 was successfully used to make chimeric mice. Germline transmission was accomplished. J:283611
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/03/2022
MGI 6.21
The Jackson Laboratory