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Spns2tm1a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Spns2tm1a(KOMP)Wtsi
Name: spinster homolog 2; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4460276
Synonyms: Spns2tr
Gene: Spns2  Location: Chr11:72451638-72489904 bp, - strand  Genetic Position: Chr11, 44.31 cM
Mutation
origin
Mutant Cell Lines:  EPD0090_5_A05, EPD0090_5_B04, EPD0090_5_C04, EPD0090_5_D06, EPD0090_5_E04, EPD0090_5_E05, EPD0090_5_E06, EPD0090_5_F04, EPD0090_5_F05, EPD0090_5_G04
Germline Transmission:  Earliest citation of germline transmission: J:188933
Parent Cell Line:  JM8.N19 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 72464268 of Chromosome 11 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 72463407. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:188933)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 12 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Spns2 Mutation:  12 strains or lines available
Notes
Cell line EPD0090_5_B04 was successfully used to make chimeric mice. Germline transmission was accomplished. J:188933
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
06/12/2018
MGI 6.12
The Jackson Laboratory