Tg(tetO-Creb1*)7941Nes
Transgene Detail

Summary

• Symbol: Tg(tetO-Creb1*)7941Nes
• Name: transgene insertion 7941, Eric Nestler
• MGI ID: MGI:4460255
• Synonyms: TetOp-mCREB, TetOp-mCREB line 9
• Transgene: Tg(tetO-Creb1*)7941Nes Location: unknown
• Alliance: Tg(tetO-Creb1*)7941Nes page

Transgene origin

• Strain of Origin: SJL/J x C57BL/6J

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: This transgene contains the tet operon (tetO) and a minimal CMV promoter controlling expression of a cDNA encoding a dominant-negative mutant rat cAMP-responsive element binding protein 1 (CREB1), alpha isoform, bearing a FLAG peptide tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). The coding sequence is followed by the SV40 intron and polyadenylation signal. The mutant CREB1 has alanine in place of the critical regulatory serine at position 133, eliminating the phosphorylation site. Unphosphorylated CREB binds to cAMP response elements (CREs), blocking access by wild-type CRE and other CRE-binding proteins. (J:128618)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:92942 McClung CA, et al., Regulation of gene expression and cocaine reward by CREB and DeltaFosB. Nat Neurosci. 2003 Nov;6(11):1208-15
• All: 3 reference(s)