4833439L19Rik^{tm2a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: 4833439L19Rik^{tm2a(EUCOMM)Hmgu}
• Name: RIKEN cDNA 4833439L19 gene; targeted mutation 2a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4457532
• Gene: 4833439L19Rik Location: Chr13:54699031-54713195 bp, - strand Genetic Position: Chr13, 28.79 cM
• IMPC: 4833439L19Rik gene page

Mutation origin

• Mutant Cell Lines: HEPD0640_4_A03, HEPD0640_4_A04, HEPD0640_4_A05, HEPD0640_4_A06, HEPD0640_4_B02, HEPD0640_4_B03, HEPD0640_4_B04, HEPD0640_4_B06, HEPD0640_4_C01, HEPD0640_4_C02, HEPD0640_4_C03, HEPD0640_4_C06, HEPD0640_4_D01, HEPD0640_4_D03, HEPD0640_4_D06, HEPD0640_4_E02, HEPD0640_4_F04, HEPD0640_4_G04, HEPD0640_4_G05, HEPD0640_4_G06, HEPD0640_4_H02, HEPD0640_4_H03, HEPD0640_4_H05, HEPD0640_4_H06
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 54704118 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 54704886. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any 4833439L19Rik Mutation: 12 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)