Clcn5^tm2.1Tjj
Targeted Allele Detail

Summary

• Symbol: Clcn5^tm2.1Tjj
• Name: chloride channel, voltage-sensitive 5; targeted mutation 2.1, Thomas J Jentsch
• MGI ID: MGI:4457504
• Synonyms: Clcn5^unc
• Gene: Clcn5 Location: ChrX:7020049-7185597 bp, - strand Genetic Position: ChrX, 3.21 cM, cytoband F3-F4
• Alliance: Clcn5^tm2.1Tjj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:160701
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: Exon 6 was replaced with one in which nucleotide substitution (A to C) result in the amino acid substitution of alanine for glutamic acid at position 211 (E211A). A floxed neo cassette inserted downstream of the modified neo cassette was removed by cre mediated recombination. This substitution results converts the Cl-/H+ transporter into a Cl- conductor (uncoupled). (J:160701)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clcn5 Mutation: 16 strains or lines available

References

• Original: J:160701 Novarino G, et al., Endosomal chloride-proton exchange rather than chloride conductance is crucial for renal endocytosis. Science. 2010 Jun 11;328(5984):1398-401
• All: 1 reference(s)