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Ros1tm1a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Ros1tm1a(KOMP)Wtsi
Name: Ros1 proto-oncogene; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4456658
Gene: Ros1  Location: Chr10:51921817-52071340 bp, - strand  Genetic Position: Chr10, 26.18 cM
Mutation
origin
Mutant Cell Lines:  EPD0567_2_A01, EPD0567_2_A02, EPD0567_2_A03, EPD0567_2_A04, EPD0567_2_B01, EPD0567_2_B02, EPD0567_2_B03, EPD0567_2_B04, EPD0567_2_C03, EPD0567_2_C04, EPD0567_2_D03, EPD0567_2_D04, EPD0567_2_E01, EPD0567_2_E03, EPD0567_2_F01, EPD0567_2_F02, EPD0567_2_F03, EPD0567_2_F04, EPD0567_2_G03, EPD0567_2_H03, EPD0567_2_H04
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 52048679 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 52049509. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Ros1 Mutation:  87 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/03/2022
MGI 6.21
The Jackson Laboratory