Cby3^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cby3^{tm1a(EUCOMM)Hmgu}
• Name: chibby family member 3; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4455818
• Gene: Cby3 Location: Chr11:50248588-50250526 bp, + strand Genetic Position: Chr11, 30.54 cM, cytoband B1.2
• Alliance: Cby3^{tm1a(EUCOMM)Hmgu} page
• IMPC: Cby3 gene page

Mutation origin

• Mutant Cell Lines: HEPD0619_6_C03, HEPD0619_6_E04, HEPD0619_6_H03, HEPD0623_3_A05, HEPD0634_4_A05, HEPD0634_4_A07, HEPD0634_4_B06, HEPD0634_4_C08, HEPD0634_4_D08, HEPD0634_4_E08, HEPD0634_4_F05, HEPD0634_4_G05, HEPD0634_4_G06
• Germline Transmission: Earliest citation of germline transmission: J:344681
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 50249413 of Chromosome 11 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 50250763. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157065, J:344681)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cby3 Mutation: 9 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)