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Mmp12tm1a(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Mmp12tm1a(EUCOMM)Hmgu
Name: matrix metallopeptidase 12; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4455797
Gene: Mmp12  Location: Chr9:7344381-7369499 bp, + strand  Genetic Position: Chr9, 2.46 cM
Mutation
origin
Mutant Cell Lines:  HEPD0634_1_B03, HEPD0634_1_C04, HEPD0634_1_D03, HEPD0634_1_E01, HEPD0634_1_E02, HEPD0634_1_E04, HEPD0634_1_F03, HEPD0634_1_F04, HEPD0634_1_H02, HEPD0634_1_H03, HEPD0654_5_A02, HEPD0654_5_B02, HEPD0654_5_B04, HEPD0654_5_E02, HEPD0654_5_E03, HEPD0654_5_F03, HEPD0654_5_F04, HEPD0654_5_G02, HEPD0654_5_G03, HEPD0654_5_H03 (Helmholtz Zentrum Muenchen GmbH)
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 7348164 of Chromosome 9 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 7349122. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:157065)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Mmp12 Mutation:  16 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/21/2017
MGI 6.08
The Jackson Laboratory