Pigb^{tm1e(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Pigb^{tm1e(KOMP)Mbp}
• Name: phosphatidylinositol glycan anchor biosynthesis, class B; targeted mutation 1e, Mouse Biology Program, UC Davis
• MGI ID: MGI:4455445
• Gene: Pigb Location: Chr9:72920639-72946973 bp, - strand Genetic Position: Chr9, 40.08 cM, cytoband D
• IMPC: Pigb gene page

Mutation origin

• Mutant Cell Lines: DEPD00522_1_A06, DEPD00522_1_B08, DEPD00522_1_C07, DEPD00522_1_C08, DEPD00522_1_E05, DEPD00522_1_F08, DEPD00522_1_G05, DEPD00522_1_H05, DEPD00522_1_H06, DEPD00522_1_H08
• Germline Transmission: Unknown
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 72942296 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pigb Mutation: 23 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 1 reference(s)