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Tg(Pvalb-COP4*H134R/EYFP)15Gfng
Transgene Detail
Nomenclature
Symbol: Tg(Pvalb-COP4*H134R/EYFP)15Gfng
Name: transgene insertion 15, Guoping Feng
MGI ID: MGI:4454793
Synonyms: Prv-mhChR2-EYFP Line 15, Prv-mhChR2-YFP Line 15
Transgene: Tg(Pvalb-COP4*H134R/EYFP)15Gfng  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6 x SJL)F1
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsFirst, a channelrhodopsin-2 cDNA sequence derived from green alga Chlamydomonas reinhardtii was modified to harbor condons optimized for mammalian expression and a gain-of-function H134R substitution (CAC to CGC) designed to cause larger stationary photocurrents. This mhChR2 sequence (also called hChR2-H134R) was fused in-frame to the amino terminus of an enhanced yellow fluorescent protein sequence (EYFP) via a Not1 site with GCGGCCGCC linker sequence. This mhChR2::YFP fusion protein (also called hChR2-H134R-EYFP) sequence was inserted into the coding region of the parvalbumin (Pvalb or Prv) locus on the RP23-305H12 bacterial artificial chromosome (BAC) via homologous recombination. Mice from line 15 exhibit high EGFP expression in cerebellum, medium EGFP expression in reticular thalamic nuclei, and low EGFP expression in brainstem. (J:160657)
Phenotypes
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:160657 Feng G, Direct submission for Tg(Pvalb-COP4*H134R/EYFP)15Gfng. MGI Direct Data Submission. 2010;
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/04/2020
MGI 6.15
The Jackson Laboratory