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Tg(Thy1-hop/EYFP)2Gfng
Transgene Detail
Nomenclature
Symbol: Tg(Thy1-hop/EYFP)2Gfng
Name: transgene insertion 2, Guoping Feng
MGI ID: MGI:4454787
Synonyms: Thy1-enPHR-ERD Line 2, Thy1-eNpHR-YFP line 2
Transgene: Tg(Thy1-hop/EYFP)2Gfng  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6 x SJL)F1
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsA fusion protein was created with the halophilic bacterium Natronobacterium pharaonisfunctions halorhodopsin (NpHR) fused in-frame with an enhanced yellow fluorescent protein (EYFP). The fusion protein was optimized for expression in mammalian systems and enhanced membrane translocation/reduced bleb formation via C-terminal addition of an endoplasmic reticulum exporting sequence (amino acids 373 to 385 from the potassium channel Kir2.1 gene [NANSFCYENEVALTSK]). This enhanced fusion protein (eNpHR::YFP) was placed downstream of the modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns), and followed by a bovine growth hormone polyA signal. Mice derived from founder line 2 exhibit high expression of EYFP in layer 5 pyramidal neurons at cortex, dentate gyrus, thalamus, superior colliculus, inferior culliculus, brainstem, amydagala and cerebellum. (J:160661)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:160661 Feng G, DDS for Tg(Thy1-HOP/EYFP)2Gfng. MGI Direct Data Submission. 2010;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/04/2020
MGI 6.15
The Jackson Laboratory