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Symbol: |
Shank3tm1a(KOMP)Mbp |
Name: |
SH3 and multiple ankyrin repeat domains 3; targeted mutation 1a, Mouse Biology Program, UC Davis |
MGI ID: |
MGI:4454002 |
Gene: |
Shank3 Location: Chr15:89383826-89444464 bp, + strand Genetic Position: Chr15, 44.96 cM
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Alliance: |
Shank3tm1a(KOMP)Mbp page
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IMPC: |
Shank3 gene page |
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Mutant Cell Lines: |
DEPD00516_3_A07, DEPD00516_3_B09, DEPD00516_3_B11, DEPD00516_3_B12, DEPD00516_3_C07, DEPD00516_3_C08, DEPD00516_3_C09, DEPD00516_3_C10, DEPD00516_3_C12, DEPD00516_3_D07, DEPD00516_3_D09, DEPD00516_3_E07, DEPD00516_3_E08, DEPD00516_3_E09, DEPD00516_3_E10, DEPD00516_3_E11, DEPD00516_3_E12, DEPD00516_3_F07, DEPD00516_3_F08, DEPD00516_3_F09, DEPD00516_3_F11, DEPD00516_3_F12, DEPD00516_3_G07, DEPD00516_3_H07, DEPD00516_3_H08, DEPD00516_3_H09, DEPD00516_3_H10 |
Germline Transmission: |
Earliest citation of germline transmission:
J:233405
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Parent Cell Line: |
JM8A1.N3 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Pgk_P
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Mutation details: The L1L2_Pgk_P cassette was inserted at position 89389112 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 89389949. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157064)
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Cell line DEPD00516_3_A07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:233405
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Original: |
J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010; |
All: |
2 reference(s) |
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