Tg(CAG-flpo)1Afst
Transgene Detail

Summary

• Symbol: Tg(CAG-flpo)1Afst
• Name: transgene insertion 1, A Francis Stewart
• MGI ID: MGI:4453967
• Synonyms: CAGGs-Flpo, Flpo
• Transgene: Tg(CAG-flpo)1Afst Location: unknown
• Alliance: Tg(CAG-flpo)1Afst page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:160215
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(CAG-flpo)1Afst expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: A linearized plasmid encoding the CAGGs promoter driving a codon optimized Flp recombinase (Flpo) and an IRES-puromycin sequence was introduced into JM8.F6 ES cells. Two clones were injected into 8-cell C57BL/6 albino embryos. One chimera showed germline transmission when crossed to C57BL/6 albino animals. (J:160215)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

References

• Original: J:160215 Kranz A, et al., An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase. Genesis. 2010 Aug;48(8):512-20
• All: 60 reference(s)