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Targeted Allele Detail
Symbol: Arhgap35tm2Jset
Name: Rho GTPase activating protein 35; targeted mutation 2, Jeffrey Settleman
MGI ID: MGI:4453472
Synonyms: p190RhoGAP null
Gene: Arhgap35  Location: Chr7:16494473-16615244 bp, - strand  Genetic Position: Chr7, 9.15 cM
Germline Transmission:  Earliest citation of germline transmission: J:160127
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe aim was to generate a novel mouse strain with a complete deletion of the p190RhoGAP-encoding gene. 1.5 kb of sequence, including the start codon and encoding the GTPase domain of exon 1, was replaced by a promoterless selection-reporter cassette encoding a Beta-Galactosidase-Neomycin resistance fusion protein in the sense orientation. The insertion site of the targeting vector is between a BamHI restriction site upstream of exon 1 and an XbaI site at the end of the replaced 1.5 kb portion. The Beta-Geo fusion protein expression is totally dependent on the endogenous promoter. The disrupted gene does not have any detectable protein products. The primary sequence of the mutant allele was depositied in the GenBank database (www.ncbi.nlm.nih.gov/genbank) under accession number HM365221. (J:162994)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Arhgap35 Mutation:  31 strains or lines available
Original:  J:160127 Nemeth T, Targeted mutation of the glucocorticoid receptor DNA binding factor 1. MGI Direct Data Submission. 2010;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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