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Rnf169tm1a(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Rnf169tm1a(EUCOMM)Hmgu
Name: ring finger protein 169; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4452734
Gene: Rnf169  Location: Chr7:99920254-99980448 bp, - strand  Genetic Position: Chr7, 54.22 cM
Mutation
origin
Mutant Cell Lines:  HEPD0619_1_A02, HEPD0619_1_B01, HEPD0619_1_B04, HEPD0619_1_C01, HEPD0619_1_D01, HEPD0619_1_E04, HEPD0619_1_F03, HEPD0619_1_F04, HEPD0619_1_G03, HEPD0619_1_G04, HEPD0619_1_H01, HEPD0619_1_H04, HEPD0634_3_A05, HEPD0634_3_A06, HEPD0634_3_A07, HEPD0634_3_B06, HEPD0634_3_B07, HEPD0634_3_B08, HEPD0634_3_C07, HEPD0634_3_C08, HEPD0634_3_D05, HEPD0634_3_D07, HEPD0634_3_D08, HEPD0634_3_E06, HEPD0634_3_E08, HEPD0634_3_F05, HEPD0634_3_F06, HEPD0634_3_F07, HEPD0634_3_G05, HEPD0634_3_H07
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 99935758 of Chromosome 7 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 99934871. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Rnf169 Mutation:  22 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/16/2021
MGI 6.16
The Jackson Laboratory