Spout1^{tm1e(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Spout1^{tm1e(EUCOMM)Hmgu}
• Name: SPOUT domain containing methyltransferase 1; targeted mutation 1e, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4451825
• Gene: Spout1 Location: Chr2:30063465-30068471 bp, - strand Genetic Position: Chr2, 21.29 cM
• IMPC: Spout1 gene page

Mutation origin

• Mutant Cell Lines: HEPD0615_3_B05, HEPD0615_3_B07, HEPD0615_3_B08, HEPD0615_3_C05, HEPD0615_3_C06, HEPD0615_3_C07, HEPD0615_3_C08, HEPD0615_3_D05, HEPD0615_3_D06, HEPD0615_3_D07, HEPD0615_3_E08, HEPD0615_3_F05, HEPD0615_3_F08, HEPD0615_3_G06, HEPD0615_3_G08, HEPD0615_3_H05, HEPD0615_3_H06, HEPD0615_3_H07
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30067172 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spout1 Mutation: 10 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)