Sash1tm1a(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Sash1tm1a(KOMP)Wtsi |
Name: |
SAM and SH3 domain containing 1; targeted mutation 1a, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4451401 |
Gene: |
Sash1 Location: Chr10:8597983-8761814 bp, - strand Genetic Position: Chr10, 2.75 cM
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IMPC: |
Sash1 gene page |
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Mutant Cell Lines: |
EPD0504_1_A09, EPD0504_1_B09, EPD0504_1_B10, EPD0504_1_B11, EPD0504_1_C09, EPD0504_1_D10, EPD0504_1_D11, EPD0504_1_E10, EPD0504_1_E11, EPD0504_1_E12, EPD0504_1_F12 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 8616933 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 8617784. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:148605, J:173534)
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Original: |
J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009; |
All: |
2 reference(s) |
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