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Acacbtm1.2Lowl
Targeted Allele Detail
Nomenclature
Symbol: Acacbtm1.2Lowl
Name: acetyl-Coenzyme A carboxylase beta; targeted mutation 1.2, Bradford B Lowell
MGI ID: MGI:4450934
Synonyms: Acc2KO
Gene: Acacb  Location: Chr5:114146535-114250761 bp, + strand  Genetic Position: Chr5, 55.97 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:159285
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsLoxP sites were positioned upstream of the preceding exon and downstream of the biotin binding domain exon using an Frt flanked kanamycin resistance cassette. The Frt-flanked selection cassette was removed by Flpe recombinase-mediated recombination. These two exons were deleted by Cre-mediated recombination, which introduces a frameshift and nonsense mutation after Asp865 in any translated protein. Immunoblotting showed no truncated protein expression in muscle lysates from homozygous mice. (J:159285)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Acacb Mutation:  54 strains or lines available
References
Original:  J:159285 Olson DP, et al., Gene knockout of Acc2 has little effect on body weight, fat mass, or food intake. Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7598-603
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory