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Targeted Allele Detail
Symbol: Actn4tm1a(EUCOMM)Wtsi
Name: actinin alpha 4; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4441842
Gene: Actn4  Location: Chr7:28893248-28962340 bp, - strand  Genetic Position: Chr7, 16.94 cM
Mutant Cell Lines:  EPD0525_2_A10, EPD0525_2_A12, EPD0525_2_B09, EPD0525_2_B11, EPD0525_2_B12, EPD0525_2_C10, EPD0525_2_C11, EPD0525_2_C12, EPD0525_2_D09, EPD0525_2_D10, EPD0525_2_D11, EPD0525_2_D12, EPD0525_2_E09, EPD0525_2_E10, EPD0525_2_E11, EPD0525_2_E12, EPD0525_2_F10, EPD0525_2_F11, EPD0525_2_F12, EPD0525_2_G09, EPD0525_2_G10, EPD0525_2_G11, EPD0525_2_H09
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt1
Mutation detailsThe L1L2_gt1 cassette was inserted at position 28915746 of Chromosome 7 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 28914966. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 20 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Actn4 Mutation:  45 strains or lines available
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
The Jackson Laboratory