Kif3a^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kif3a^{tm1a(EUCOMM)Wtsi}
• Name: kinesin family member 3A; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4441640
• Gene: Kif3a Location: Chr11:53458206-53492794 bp, + strand Genetic Position: Chr11, 31.97 cM, cytoband A5-B1
• IMPC: Kif3a gene page

Mutation origin

• Mutant Cell Lines: EPD0023_1_A11, EPD0023_1_A12, EPD0023_1_B10, EPD0023_1_B12, EPD0023_1_C10, EPD0023_1_C11, EPD0023_1_C12, EPD0023_1_D10, EPD0023_1_D11, EPD0023_1_E10, EPD0023_1_E11, EPD0023_1_E12, EPD0023_1_F11, EPD0023_1_F12, EPD0023_1_G10, EPD0023_1_G11, EPD0023_1_G12, EPD0023_1_H10, EPD0023_1_H11
• Germline Transmission: Unknown
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 53470334 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 53470990. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Kif3a Mutation: 31 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)