Prim1^{tm2a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Prim1^{tm2a(EUCOMM)Wtsi}
• Name: DNA primase, p49 subunit; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4441632
• Gene: Prim1 Location: Chr10:127851084-127865899 bp, + strand Genetic Position: Chr10, 76.39 cM, cytoband D3
• IMPC: Prim1 gene page

Mutation origin

• Mutant Cell Lines: EPD0499_1_A06, EPD0499_1_C06, EPD0499_1_C07, EPD0499_1_D08, EPD0499_1_E06, EPD0499_1_G05, EPD0509_4_A03, EPD0509_4_B01, EPD0509_4_B03, EPD0509_4_B04, EPD0509_4_C02, EPD0509_4_C04, EPD0509_4_E01, EPD0509_4_E04, EPD0509_4_F03, EPD0509_4_G01, EPD0509_4_H02, EPD0509_4_H03, EPD0509_4_H04
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 127855535 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 127857836. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prim1 Mutation: 25 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)