Hprt1tm1(tetO-lacZ)Frje
Targeted Allele Detail
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Symbol: |
Hprt1tm1(tetO-lacZ)Frje |
Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Frederic Jaisser |
MGI ID: |
MGI:4437604 |
Synonyms: |
GR-tetO-LacZ |
Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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Alliance: |
Hprt1tm1(tetO-lacZ)Frje page
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Germline Transmission: |
Earliest citation of germline transmission:
J:157969
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Parent Cell Line: |
Other (see notes) (ES Cell)
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Strain of Origin: |
129 and C57BL/6
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Allele Type: |
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Targeted (Inducible, Null/knockout, Reporter) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Mutation details: A transgene containing a bidirectional tetracycline promoter (tetO) driving expression of human GR1 (5' of tetO) and lacZ (3' of tetO) was inserted into the locus by homologous recombination. Selection was achieved by restoring the locus that is defective in the ES cells used to generate this allele. No GR1 expression was detected following activation of tetO where as lacZ expression is detected.
(J:157969)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Hprt1 Mutation: |
1272 strains or lines available
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ES cell line = BPES11 (carries Hprtb-m3). This cell line was established from Hprt mutant mice on a highly mixed C57BL/6 and 129 background that had been derived from ES cell line BK4 (129P2/OlaHsd).
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Original: |
J:157969 Palais G, et al., Targeted transgenesis at the HPRT locus: an efficient strategy to achieve tightly controlled in vivo conditional expression with the tet system. Physiol Genomics. 2009 Apr 10;37(2):140-6 |
All: |
1 reference(s) |
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