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Hprt1tm1(tetO-lacZ)Frje
Targeted Allele Detail
Summary
Symbol: Hprt1tm1(tetO-lacZ)Frje
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Frederic Jaisser
MGI ID: MGI:4437604
Synonyms: GR-tetO-LacZ
Gene: Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance: Hprt1tm1(tetO-lacZ)Frje page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:157969
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129 and C57BL/6
Mutation
description
Allele Type:    Targeted (Inducible, Null/knockout, Reporter)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
 
Mutation detailsA transgene containing a bidirectional tetracycline promoter (tetO) driving expression of human GR1 (5' of tetO) and lacZ (3' of tetO) was inserted into the locus by homologous recombination. Selection was achieved by restoring the locus that is defective in the ES cells used to generate this allele. No GR1 expression was detected following activation of tetO where as lacZ expression is detected. (J:157969)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:  1272 strains or lines available
Notes
ES cell line = BPES11 (carries Hprtb-m3). This cell line was established from Hprt mutant mice on a highly mixed C57BL/6 and 129 background that had been derived from ES cell line BK4 (129P2/OlaHsd).
References
Original:  J:157969 Palais G, et al., Targeted transgenesis at the HPRT locus: an efficient strategy to achieve tightly controlled in vivo conditional expression with the tet system. Physiol Genomics. 2009 Apr 10;37(2):140-6
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory