Bccip^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Bccip^{tm1a(EUCOMM)Hmgu}
• Name: BRCA2 and CDKN1A interacting protein; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435867
• Gene: Bccip Location: Chr7:133311062-133322874 bp, + strand Genetic Position: Chr7, 77.3 cM, cytoband F4
• Alliance: Bccip^{tm1a(EUCOMM)Hmgu} page
• IMPC: Bccip gene page

Mutation origin

• Mutant Cell Lines: HEPD0517_2_D10, HEPD0517_2_D11, HEPD0517_2_E11, HEPD0517_2_F12, HEPD0517_2_H10, HEPD0517_2_H11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 133318877 of Chromosome 7 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 5 at position 133319805. Exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bccip Mutation: 22 strains or lines available

Notes

Cell line HEPD0517_2_E11 was successfully used to make chimeric mice. Although chimeric mice were obtained, germline transmission was NOT accomplished. J:297226.

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 3 reference(s)