Entpd4b^{tm2e(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Entpd4b^{tm2e(EUCOMM)Hmgu}
• Name: ectonucleoside triphosphate diphosphohydrolase 4B; targeted mutation 2e, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435376
• Synonyms: Entpd4^{tm2e(EUCOMM)Hmgu}
• Gene: Entpd4b Location: Chr14:69792850-69822389 bp, + strand Genetic Position: Chr14, Syntenic
• IMPC: Entpd4b gene page

Mutation origin

• Mutant Cell Lines: HEPD0548_5_A03, HEPD0548_5_B03, HEPD0548_5_C02, HEPD0548_5_E01, HEPD0548_5_F02, HEPD0548_5_F04
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 69814051 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Entpd4b Mutation: 0 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)