Mdh1^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Mdh1^{tm1a(EUCOMM)Hmgu}
• Name: malate dehydrogenase 1, NAD (soluble); targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435221
• Gene: Mdh1 Location: Chr11:21506692-21521934 bp, - strand Genetic Position: Chr11, 13.89 cM
• IMPC: Mdh1 gene page

Mutation origin

• Mutant Cell Lines: HEPD0515_6_A02, HEPD0515_6_A03, HEPD0515_6_B01, HEPD0515_6_B02, HEPD0515_6_C01, HEPD0515_6_C02, HEPD0515_6_C03, HEPD0515_6_D01, HEPD0515_6_D02, HEPD0515_6_D03, HEPD0515_6_E01, HEPD0515_6_E02, HEPD0515_6_E03, HEPD0515_6_F01, HEPD0515_6_F02, HEPD0515_6_F03, HEPD0515_6_G01, HEPD0515_6_H01, HEPD0515_6_H02
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 21515523 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 21516263. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mdh1 Mutation: 35 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)