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Targeted Allele Detail
Symbol: Bloc1s1tm1a(EUCOMM)Hmgu
Name: biogenesis of lysosomal organelles complex-1, subunit 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4434990
Synonyms: GCN5L1-, GCN5L1tm1a
Gene: Bloc1s1  Location: Chr10:128919914-128923524 bp, - strand  Genetic Position: Chr10, 77.19 cM
Mutant Cell Lines:  HEPD0550_3_A10, HEPD0550_3_B10, HEPD0550_3_B11, HEPD0550_3_C09, HEPD0550_3_D09, HEPD0550_3_E10, HEPD0550_3_E11, HEPD0550_3_E12, HEPD0550_3_F09, HEPD0550_3_F12, HEPD0550_3_G11, HEPD0550_3_H10, HEPD0570_2_A07, HEPD0570_2_A10, HEPD0570_2_B07, HEPD0570_2_C08, HEPD0570_2_D08, HEPD0570_2_E12, HEPD0570_2_F08, HEPD0570_2_F09, HEPD0570_2_G07, HEPD0570_2_G11, HEPD0570_2_H11
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8A1.N3 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 128921140 of Chromosome 10 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 128920408. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Bloc1s1 Mutation:  1 strain or line available
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
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