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Mtrf1tm1a(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Mtrf1tm1a(EUCOMM)Hmgu
Name: mitochondrial translational release factor 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4434932
Gene: Mtrf1  Location: Chr14:79397772-79423650 bp, + strand  Genetic Position: Chr14, 42.63 cM
Mutation
origin
Mutant Cell Lines:  HEPD0530_4_A01, HEPD0530_4_B02, HEPD0530_4_B04, HEPD0530_4_D02, HEPD0530_4_D03, HEPD0530_4_E02, HEPD0530_4_F01, HEPD0530_4_F02, HEPD0530_4_F03, HEPD0530_4_G01, HEPD0530_4_H01, HEPD0530_4_H03, HEPD0530_4_H04 (Helmholtz Zentrum Muenchen GmbH)
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Pgk_P
 
Mutation detailsThe L1L2_Pgk_P cassette was inserted at position 79402335 of Chromosome 14 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 79403136. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:157065)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Mtrf1 Mutation:  13 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/21/2017
MGI 6.08
The Jackson Laboratory