Polr3h^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Polr3h^{tm1a(EUCOMM)Hmgu}
• Name: polymerase (RNA) III (DNA directed) polypeptide H; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4434878
• Gene: Polr3h Location: Chr15:81799231-81813824 bp, - strand Genetic Position: Chr15, 38.26 cM, cytoband E2
• IMPC: Polr3h gene page

Mutation origin

• Mutant Cell Lines: HEPD0528_7_A02, HEPD0528_7_A03, HEPD0528_7_B01, HEPD0528_7_B02, HEPD0528_7_B03, HEPD0528_7_B05, HEPD0528_7_C02, HEPD0528_7_C03, HEPD0528_7_C06, HEPD0528_7_D04, HEPD0528_7_D05, HEPD0528_7_D06, HEPD0528_7_E01, HEPD0528_7_E02, HEPD0528_7_E03, HEPD0528_7_E05, HEPD0528_7_E06, HEPD0528_7_F02, HEPD0528_7_F04, HEPD0528_7_F06, HEPD0528_7_G01, HEPD0528_7_G05, HEPD0528_7_G06, HEPD0528_7_H01, HEPD0528_7_H04
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 81802178 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 81803386. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polr3h Mutation: 19 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)