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Pef1tm1a(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Pef1tm1a(EUCOMM)Hmgu
Name: penta-EF hand domain containing 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4434599
Gene: Pef1  Location: Chr4:130001349-130021927 bp, + strand  Genetic Position: Chr4, 63.43 cM, cytoband D3
Alliance: Pef1tm1a(EUCOMM)Hmgu page
IMPC: Pef1 gene page
Mutation
origin
Mutant Cell Lines:  HEPD0074_4_B06, HEPD0074_4_C04, HEPD0074_4_C05, HEPD0074_4_E04, HEPD0074_4_F04, HEPD0074_4_F05, HEPD0074_4_F06, HEPD0074_4_G04, HEPD0074_4_G05, HEPD0074_4_H04, HEPD0074_4_H06
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 130014500 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 130015513. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Pef1 Mutation:  15 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory