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Targeted Allele Detail
Symbol: Pik3c3tm1a(EUCOMM)Wtsi
Name: phosphoinositide-3-kinase, class 3; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4433098
Gene: Pik3c3  Location: Chr18:30272747-30348126 bp, + strand  Genetic Position: Chr18, 17.41 cM
Mutant Cell Lines:  EPD0103_4_A07, EPD0103_4_B07, EPD0103_4_B08, EPD0103_4_D07, EPD0103_4_D09, EPD0103_4_E08, EPD0103_4_F09, EPD0103_4_G08, EPD0103_4_G09, EPD0103_4_H09 (Wellcome Trust Sanger Institute)
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt2
Mutation detailsThe L1L2_gt2 cassette was inserted at position 30281495 of Chromosome 18 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 30282336. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:155845, J:173534)
Generation of the Pik3c3tm1a(EUCOMM)Wtsi and Pik3c3tm1c(EUCOMM)Wtsi alleles
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pik3c3 Mutation:  22 strains or lines available
Cell line EPD0103_4_A07 was used to make chimeric mice. Germline transmission was accomplished. J:182628
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  4 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.08
The Jackson Laboratory