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Mks1tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Mks1tm1a(EUCOMM)Wtsi
Name: Meckel syndrome, type 1; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4433029
Gene: Mks1  Location: Chr11:87853215-87863803 bp, + strand  Genetic Position: Chr11, 52.24 cM
Mutation
origin
Mutant Cell Lines:  EPD0143_4_A03, EPD0225_12_A07, EPD0225_12_A08, EPD0225_12_C04, EPD0225_12_C07, EPD0225_12_D09, EPD0225_12_F08
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 87856109 of Chromosome 11 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 87857574. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:196290)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 28 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Mks1 Mutation:  3 strains or lines available
Notes
Cell line EPD0143_4_A03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:196290
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/10/2019
MGI 6.14
The Jackson Laboratory