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Targeted Allele Detail
Symbol: Crls1tm1a(EUCOMM)Wtsi
Name: cardiolipin synthase 1; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4432552
Gene: Crls1  Location: Chr2:132688586-132708705 bp, + strand  Genetic Position: Chr2, 64.77 cM, cytoband F3
Mutant Cell Lines:  EPD0423_7_A05, EPD0423_7_A07, EPD0423_7_B06, EPD0423_7_B07, EPD0423_7_C05, EPD0423_7_C07, EPD0423_7_C08, EPD0423_7_D05, EPD0423_7_D06, EPD0423_7_D08, EPD0423_7_E05, EPD0423_7_F05, EPD0423_7_F07, EPD0423_7_F08, EPD0423_7_G05, EPD0423_7_H08
Germline Transmission:  Earliest citation of germline transmission: J:261316
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 132696392 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 132697184. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Crls1 Mutation:  9 strains or lines available
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory