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Sptbn1tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Sptbn1tm1a(EUCOMM)Wtsi
Name: spectrin beta, non-erythrocytic 1; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4432393
Synonyms: Spnb2tm1a, Spnb2tm1a(EUCOMM)Wtsi
Gene: Sptbn1  Location: Chr11:30099395-30268175 bp, - strand  Genetic Position: Chr11, 17.44 cM
Mutation
origin
Mutant Cell Lines:  EPD0065_1_A10, EPD0065_1_B10, EPD0065_1_B12, EPD0065_1_C11, EPD0065_1_D10, EPD0065_1_D11, EPD0065_1_D12, EPD0065_1_E10, EPD0065_1_E11, EPD0065_1_F10, EPD0065_1_F11, EPD0065_1_F12, EPD0065_1_G10, EPD0065_1_G11, EPD0065_1_G12, EPD0065_1_H11, EPD0065_1_H12
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt1
 
Mutation detailsThe L1L2_gt1 cassette was inserted at position 30159932 of Chromosome 11 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 30159028. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 7 assay results
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Sptbn1 Mutation:  33 strains or lines available
Notes
Cell line EPD0065_1_F10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:200082
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/14/2019
MGI 6.14
The Jackson Laboratory