Hdxtm1a(EUCOMM)Wtsi
Targeted Allele Detail
|
Symbol: |
Hdxtm1a(EUCOMM)Wtsi |
Name: |
highly divergent homeobox; targeted mutation 1a, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4432202 |
Gene: |
Hdx Location: ChrX:110484901-110606908 bp, - strand Genetic Position: ChrX, 48.29 cM
|
Alliance: |
Hdxtm1a(EUCOMM)Wtsi page
|
IMPC: |
Hdx gene page |
|
Mutant Cell Lines: |
EPD0203_3_A11, EPD0203_3_B10, EPD0203_3_C09, EPD0203_3_C10, EPD0203_3_C11, EPD0203_3_D11, EPD0203_3_G09, EPD0203_3_G10, EPD0203_3_G11, EPD0203_3_G12 |
Germline Transmission: |
Earliest citation of germline transmission:
J:188991
|
Parent Cell Line: |
JM8.N4 (ES Cell)
|
Strain of Origin: |
C57BL/6N
|
Project Collection: |
EUCOMM
|
|
Allele Type: |
|
Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
|
Insertion
Vector: L1L2_Bact_P
|
|
|
Mutation details: The L1L2_Bact_P cassette was inserted at position 110510030 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 110511000. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:155845, J:173534)
|
|
|
|
Original: |
J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010; |
All: |
3 reference(s) |
|